WebBarth A. and Corrie J. E. (2002) Characterization of a new caged proton capable of inducing large pH jumps. Biophys. J. 83, 2864–2871. Abstract. Ultraviolet/visible (UV/vis) absorption spectroscopy is a powerful tool for steady-state and time-resolved studies of protein-ligand interactions. Prosthetic groups in proteins frequently have strong ... WebFeb 11, 2024 · Keywords: bioenergetics, local proton circuit, proton transport, fluorimetry, caged proton, interfacial proton diffusion. Go to: 1. Introduction. Interfacial proton diffusion is a widespread phenomenon. It is known as spillover in catalysis, where protons are created on a metal surface and migrate to the surface of the support [1,2].
NPE-caged-proton Supplier CAS 1186195-63-0 - Tocris …
WebDec 1, 2014 · First of all, we carried out several dynamic proton NMR measurements at different temperatures with a 1:1 molar ratio of reactants using 2 mol% of catalyst relative … WebNPE-caged-proton is a 1-(2-nitrophenyl)ethyl caged proton that releases a proton and a sulfate ion upon photolysis at 350 - 355 nm. Generates rapid acidifications (pH jumps) down to pH 2. Technical Data. M.Wt: 269.21. Formula: C 8 H 8 NNaO 6 S. Solubility: Soluble to 100 mM in water and to 100 mM in DMSO. Purity: ≥98%. Storage: shortest alaska airlines flight
Kinetics of Proton Binding to the Sarcoplasmic ... - ScienceDirect
WebNPE-caged-proton is a 1- (2-nitrophenyl)ethyl caged proton that releases a proton and a sulfate ion upon photolysis. Generates rapid acidifications down to pH 2 (pH jumps). … WebNov 1, 2002 · A new caged proton, 2-methoxy-5-nitrophenyl sulfate, was synthesized and used in time-resolved pH jump experiments to study proton binding in the sarcoplasmic … WebApr 1, 2012 · A photoactivatable caged cyanide, 1- (2-nitrophenyl)ethyl (NPE) cyanide, was synthesized, which upon irradiation in the near UV releases cyanide. It is demonstrated that the compound can be used to induce formation of the Fe III –CN - complex in the heme protein nitrophorin 4 from Rhodnius prolixus. S. R. Adams, R. Y. Tsien, Controlling cell ... san francisco state university spring break