WebChip It Express Enzymatic Magnetic Chromatin Immunoprecipitation Kit Enzymatic Shearing Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: … WebMar 1, 2024 · The chromatin immunoprecipitation (ChIP) assay was performed using the ChIP-IT Express Enzymatic Kit (Active Motif, 53009) as per the manufacturer's instructions. The chromatin was sheared using the ChIP-IT Express Enzymatic Kit (Active Motif, 53035). We used Histone H3ac (pan-acetyl) antibody (Active Motif, 39139), Histone …
(53009) ChIP-IT® Express Enzymatic - Active Motif - CiteAb
WebApr 21, 2024 · Crosslinked DNA-proteins were fragmented with ChIP-IT® Express Enzymatic Shearing Kit (Active Motif),cell lysates were further subject to IP with antibodies targeting specific protein. ... DNA was purified and subjected to library preparation. ChIP-seq library preparation kit (Vazyme, catlog: ND607-01) was used for preparation of ChIP-seq ... WebChIP-IT Kits provide all of the critical components needed for successful ChIP in a single kit. ChIP-IT comes complete with positive & negative control antibodies and PCR primers, … huntsman\\u0027s-cup f0
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WebJan 1, 2014 · The ChIP protocol that follows is based on that of the ChIP-IT Express Enzymatic kit (Active motif) with minor modifications. Combine 10 μl of ChIP buffer 1, 1 μl of the protease inhibitor cocktail, 30 μg of the antibody, 25 μl of protein G magnetic beads, 30 μg of chromatin (which was sheared using the enzymatic shearing cocktail), and ... WebChIP-IT Express Kits utilize magnetic protein G-coated beads, which improves on traditional ChIP by reducing or eliminating several time-consuming steps. The magnetic beads … WebFeb 20, 2024 · ChIP assay was performed by using ChIP‐IT Express Enzymatic kit (Active Motif) according to the manufacturer's instructions. Briefly, cells were cross‐linked with 1% formaldehyde for 10 min, quenched with glycine followed by nuclear lysis. After isolating nuclear fractions, chromatin was enzymatically sheared into 200–100 bp. ... huntsman\u0027s-cup f4