Fluorescein diphosphate
WebJun 10, 1996 · A new fluorogenic phosphatase assay requiring no prelabeling has been developed to quantitate cell number, which can thus serve as the basis for quantitating cell adhesion or migration. The assay uses the non-fluorescent substrate 3,6-fluorescein diphosphate (FDP) whose dephosphorylation generates fluorescein. WebMar 1, 2008 · Alkaline phosphatase was chosen as a model enzyme and used to convert fluorescein diphosphate into fluorescein. Droplets of alkaline phosphatase and fluorescein diphosphate were merged and mixed on the device, resulting in a 140-nL, stopped-flow reaction chamber in which the fluorescent product was detected by a …
Fluorescein diphosphate
Did you know?
WebKey features and details. Ultrasensitive fluorogenic alkaline phosphatase substrate. CAS Number: 217305-49-2. Purity: > 98%. Soluble in DMSO and water. Form / State: Solid. … WebFluorescein diphosphate is a colorless and nonfluorescent substrate for alkaline phosphatases, sequential alkaline phosphatase mediated hydrolysis of its two phosphate …
WebJun 1, 2003 · Many assays for protein tyrosine phosphatases (PTPs) rely on colorimetric substrates or more recently developed fluorescent substrates. Enzyme activity is measured but no assessment of specific activity is made, which is important since these enzymes can exist in an enzymatically inactive form. WebFDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2* Phosphatase Substrate: 498: 517: 5 mg: 11600: FCB [Fluorescein di-beta-D-cellobioside] Cellulase …
WebFluorescein diphosphate, tetraammonium salt Description Ultrasensitive fluorogenic alkaline phosphatase substrate Biological description Ultrasensitive fluorogenic alkaline phosphatase substrate. Hydrolysis produces the green fluorescent dye, fluorescein (Ex/Em = 490/520 nm). Purity > 98% CAS Number 217305-49-2 Chemical structure Properties … WebThis addition resulted in the release and diffusion of ALP-linked anti-human IgG into the bulk solution. This event led to a 20-min single-step sandwich immunoreaction at the inner wall of capillary; the reaction was detected through the reaction with fluorescein diphosphate (FDP) which generated a fluorescent product, fluorescein.
Web人生长激素(HGH)elisa酶联免疫试剂盒价格优势： 1.专一性强。抗原与抗体的免疫反应是专一反应，而免疫酶技术以免疫反应为基础，所检测的对象是抗原（或抗体），使用的抗体除标记了酶以外，与普通抗体的免疫反应特性并无多大差别。
WebAbstract. A simple and inexpensive colorimetric assay for alkaline phosphatase (ALP) and α-fetoprotein (AFP) has been established by the hydrolysis of fluorescein diphosphate … nature based therapies horizon europeWebFluorescein diphosphate (FDP) is a fluorogenic alkaline phosphatase substrate that was first described in 19631and has been isolated in pure form and made commercially available by Molecular Probes. nature based therapistWebTarget IC 50: 6 nM for dephosphorylation of fluorescein diphosphate by Wip1 (2-240) and 13 nM for phospho-p38 MAP kinase (Thr180) Warning. Toxicity: Standard Handling (A) Reconstitution. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. marine corps officer promotionWebFDP is a colorless and nonfluorescent substrate for alkaline phosphatases. Sequential alkaline phosphatase mediated hydrolysis of its two phosphate substituents yields … marine corps officer recruitmentWebInhibition of fluorescein-Fbg binding by underivatized Fbg. Fluorescein-Fbg was diluted with increasing amounts of under- ivatized fibrinogen, and these mixtures were then analyzed for bind- ing to ADP-stimulated platelets. The total level of Fbg in each assay was held constant at 1 mgiml. After activation and binding for 10 min marine corps officer selection officesWebJul 2, 2016 · A highly sensitive and continuous protein tyrosine phosphatase (PTPase) assay using 3,6-fluorescein diphosphate (FDP) is described. Leukocyte phosphatase … nature-based tourism meaningWebOct 21, 2014 · Plant cell cultures as valuable tools for the production of specific metabolites can be greatly improved by the application of elicitors including cyclodextrins (CDs) for enhancing the yields of the desired plant compounds. Here the effects of 2,6-dimethyl-β-cyclodextrins (DIMEB) on the production of carotenoids and quinones from Artemisia … marine corps officer promotion zones