WebMay 9, 2000 · Expression of GFP or β-galactosidase was evaluated 48 h after transfection. Wt + AD, wild-type prey with the transactivating domain; WtΔAD, wild-type prey lacking the transactivating domain; Mut + AD, prey mutant with the transactivating domain that does not interact with the bait. ( B) GB133 cells maintain OriP -containing plasmids. WebNational Center for Biotechnology Information
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WebDec 11, 2024 · We used the yeast collection of genes fused to GFP as a rapid screen for examples of feedback regulation in ribosomal proteins by overexpressing a non-regulatable version of a gene and... Web1, Place the competent cell on ice. 2. Add 1ul of 10ng pUC19 plasmid into the tube containing the cell. Flick the tube gently to mix the cell and DNA, Incubate on ice for 20 mins. 3. Heat shock at ... so with synonym
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Web1 INTRODUCTION. The green fluorescent protein (GFP) from Aequorea victoria was the first fluorescent protein (FP) to be used as a reporter and as a tool for labeling proteins, cells and tissues. 1 Multiple rounds of protein engineering have yielded improved GFP variants. Initial efforts focused on photophysical properties, and led to creation of the enhanced … WebFeb 19, 2014 · The two cell types are visually distinguishable by the different GFP expression. Both cell types express the nucleic protein Nrd1-mCherry. The scale bar is 10 µm. ... Yeast cells were captured using OT and were sequentially treated with a selective Hog1 kinase inhibitor and sorbitol. As a result, Hog1-GFP nuclear migration upon sorbitol ... WebUsing the yeast two-hybrid method with an Arabidopsis root-specific cDNA library, we identified a number of putative SCR-interacting proteins ... The extra cell layer in GFP–ND-expressing plants appears to be middle cortex, as indicated by the reduction of GFP expression from the SCR promoter (Figure 1e). Premature middle cortex formation was ... so without me you\\u0027ll be fine right