WebJul 16, 2012 · Autophagic puncta formation was observed by fluorescense microscopy. ... This study indicated that the RAW264.7 cell line stably expressing GFP-LC3 is available … WebApr 28, 2024 · Using a droplet fusion assay controlled by optical tweezers, ... After treatment with HU and AZD6738, we observed a significant increase in puncta formation in the eGFP-H1 cells only ...
Pontin arginine methylation by CARM1 is crucial for epigenetic ... - Nature
WebAug 8, 2024 · Here, we report a HaloTag (Halo)-based reporter processing assay to monitor mammalian autophagic flux. We found that Halo is sensitive to lysosomal proteolysis but becomes resistant upon ligand binding. When delivered into lysosomes by autophagy, pulse-labeled Halo-based reporters (e.g. Halo-LC3 and Halo-GFP) are proteolytically processed … WebNational Center for Biotechnology Information children\u0027s memory bible verses
Echovirus induces autophagy to promote viral replication
WebMar 30, 2024 · For colony formation assays, cells were plated into 6-well culture plates at 1000–3000 cells/well, cultured for 14 days before being fixed with 4% paraformaldehyde ... A–D The impact of ASS1 knockdown on the formation of LC3B puncta in KYSE30 cells (A) and KYSE450 cells (B) with PSMD2 overexpression. WebMar 12, 2024 · GFP::LGG-1 puncta formation was analysed in the head and seam cells of wild-type, atg-18(gk378), and epg-6(bp242) L2/3 larvae expressing the adIS2122 transgene. The scheme in ( A ) indicates the developmental larval stage (L2/3) where GFP::LGG-1 puncta assessments were conducted, and the images in ( B ) show head and mid-body … WebJun 29, 2024 · Autophagic flux assay, performed by measuring MAP1LC3 protein turnover, showed that autophagy was significantly decreased 6–8 h after CaOx ... We found that MAP1LC3 puncta formation was still intact at 1–2 d after GOX injection with rapamycin, whereas the number of MAP1LC3 puncta decreased at 1–2 d after GOX ... children\u0027s memorial hermann pediatrics katy