Toyobo competent high dh5α
Web【CC301】 DH5α Chemocompetent cell-ATG Biotechnology Co., Ltd -【CC301】 DH5α Chemocompetent cell WebElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. They may be used in procedures requiring high …
Toyobo competent high dh5α
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WebThe SIG10 5a Chemically Competent Cells share the most useful genetic elements of standard cloning strains like DH5α ™ and directly replace them in cloning protocols. They provide the performance researchers need with ease of use as only common laboratory equipment is required and provide solutions for a wide range of applications at … WebIncludes: Max Efficiency DH5α Competent Cells: 5 vials, 200μL each (total of 1mL), pUC19 DNA (0.01μg/mL): 1 vial, 100μL, S.O.C. medium: 2 bottles, 6mL each Description …
WebNov 28, 2009 · The resulting vectors were used for transformation of competent high DH5α (Toyobo). The plasmids from positive white colonies were purified by QuickLyse Miniprep Kit (Qiagen). Both products of (1) and (2) were sequenced with ABI PRISM 310 DNA Analyzer or ABI PRISM 3130 DNA Analyzer (PE Biosystems, Foster City, CA) using a BigDye Terminar … WebJul 22, 2016 · The resulting plasmid DNA was used to transform Competent High DH5α (Toyobo, Tokyo, Japan) according to the manufacturer's instructions, and the transformed E. coli cells were subsequently spread onto LB agar plates containing 50 μg/ml ampicillin. The plates were incubated overnight at 37°C, and colonies resistant to ampicillin were selected.
WebApr 15, 2024 · 东洋纺TOYOBO荧光定量THUNDERBIRD qPCR Mix; ... (High Throughput) EpiXtract 总组蛋白抽提 试剂盒 （高通量） 品牌：Enzo; 康宁corning 430756 FLTR SYS,250ML,.2UM,CN,S,IND,1 真空过滤系统 250ml 0.2umCN（硝酸纤维素）膜 50mm直径 灭菌 单个包装(cf 430186) 1个/包 ... E.coli DH5α Competent Cells酶试剂盒 ...
WebMar 19, 2014 · The mixed solution was diluted 20× with H 2 O for the transformation of competent cells (TOYOBO, Competent high DH5α). 1 μL of the diluted solution and 10 μL of thawed competent cells were added to an ice-cold tube and the mixture was left on ice for 30 minutes. After heat-shock at 42°C for 45 seconds, SOC medium (200 μL) was added to … gáz plomba leszedéseWebPlasmid DNA constructs were transformed into high efficiency DH5α competent cells (NEB) for propagation, and purified by the GeneJET plasmid miniprep kit (Thermo-Scientific Rockford, IL, USA), following the manufacturer’s instructions. For cell transfection, RAW264.7 cells were transfected with PRKCB-REP, an empty REP vector using TurboFect ... gáz plombaWebProvided is an isolated binding protein containing a Taq DNA polymerase antigen-binding domain. The preparation, application and the like of the binding protein are studied. The binding protein has strong activity, has high affinity with a Taq DNA polymerase, and can be widely used in the field of PCR detection. gáz plusz gáz kftWeb高亲和性K+转运蛋白(High Affinity K+Transporter，HKT)是植物遭受盐胁迫伤害时，提高植物耐盐的关键[6-7]。Schachtman和Schroeder首次从小麦(Triticum aestivum)中克隆得到TaHKT2;1蛋白[7]，随后相继在其他植物中也发现了HKT类蛋白[7-14]。 ... 4-Nos转化到E.coli Competent Cells DH5α中，将 ... gáz piros lánghttp://www.utopbio.cn/archives/date/2024/04/15 gáz piaci ára 2022WebDH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency: • Efficient DNA cloning derived from PCR, cDNA-generation reactions • recA1 marker facilitates working with difficult to transform DNA • Ideal for generation of cDNA libraries using plasmid-derived vectors • Site-directed mutagenesis Genotype gáz plumberWebNEB. 5-alpha Competent. E. coli. (High Efficiency) NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality … autonuke